INTRUSION 2 MAKU AND DNA HOW TO

, Moreover, the oxidative stress inflicted on spermatozoa during the procedure could be another factor in inducing DNA damage. The variation in temperature during the freezing-thawing procedure produces chemical and physical stresses on spermatozoa that may adversely change the lipid composition of their membrane. ,, ,, ,, Р”В¬ryodamage of human spermatozoa is thought to be multifactorial. While the primary goal of sperm freezing is to preserve male fertility, the procedure has shown adverse effects on sperm motility, functions, and DNA integrity, especially in subfertile and infertile men. For example, it is offered to store surgically retrieved sperm from azoospermic men for future use. Over the past decades, sperm cryopreservation has evolved as a regular practice in assisted reproductive technology (ART) treatments. The procedure is routinely used to preserve fertility in men who are undergoing cancer treatment, which directly affects spermatogenesis. Sperm cryopreservation refers to the storage of spermatozoa typically at РІв‚¬вЂ™196РІВРЉ, at which all biological activity is effectively paused. Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa.

INTRUSION 2 MAKU AND DNA HOW TO

How to cite this URL: Alqawasmeh OA, Zhao M, Chan CP, Leung MB, Chow KC, Agarwal N, Mak JS, Wang CC, Pang CP, Li TC, Chu WK, Chan DY. How to cite this article: Alqawasmeh OA, Zhao M, Chan CP, Leung MB, Chow KC, Agarwal N, Mak JS, Wang CC, Pang CP, Li TC, Chu WK, Chan DY. Keywords:Р’В antioxidants cryopreservation additives sperm cryopreservation sperm DNA fragmentation sperm motility In conclusion, supplementing sperm freezing media with GTE has a significant protective effect on human sperm motility and DNA integrity, which may be of clinical interest.

However, there was no significant change in the reactive oxygen species (ROS) level. More interestingly, patients with high sperm DNA damage benefited similarly from the GTE supplementation. We found that the addition of 1.0 ng ml РІв‚¬вЂ™1 GTE to cryopreservation media significantly increased sperm motility and DNA integrity (both P < 0.05). Our data showed that GTE significantly protected sperm motility and DNA integrity against oxidative stress induced by H 2O 2when added at a final concentration of 1.0 ng ml РІв‚¬вЂ™1. After 2 weeks, the semen was thawed, and the effect on sperm motility and DNA fragmentation was observed. In part two, the semen of 45 patients was cryopreserved with or without 1.0 ng ml РІв‚¬вЂ™1 GTE. Spermatozoa were treated with GTE at different concentrations before incubation with H 2O 2. In part one, the semen of 20 normozoospermic men was used to optimize the concentration of GTE that maintains sperm motility and DNA integrity against oxidative stress, induced by hydrogen peroxide (H 2O 2). This study aims to evaluate the GTE as a potential additive in cryopreservation media of human spermatozoa. Green tea extract (GTE) is widely considered as an excellent antioxidant, and its beneficial role has been proven in other human cells. Antioxidant additives in sperm freezing media have previously brought improvements in postthawed sperm quality.

Cryopreservation impairs sperm quality and functions, including motility and DNA integrity.

Intrusion 2 maku and dna